• An image of RoSS.LN2F controlled rate freezer on transparent background, for cryopreservation and enhanced cell viability in biopharmaceutical applications.

Cryogenic freezer | RoSS.LN2F

Cryogenic freezer | RoSS.LN2F

RoSS.LN2F is a powerful cryogenic controlled rate freezer for cryopreservation down to -170°C. An enclosed and innovative platform system for dosed freezing for cell and gene therapies eliminates direct exposure and the need for mechanical compressors.

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Green icon with a cell and a snowflake on a transparent background demonstrating cell viability after freezing
Increased cell viability
Green icon of a thermometer on a transparent background demonstrating temperature control
Down to -170°C
Green icon of a water droplet and an arrow on a transparent background demonstrating scalability
Down to < 250mL/ Bag

Features and benefits of RoSS.LN2F

  • Suitable for any single-use bioprocess container*
  • Temperature range from room temperature down to -170°C
  • Fully automated & controlled
  • At highest speed & accuracy
  • Any freeze recipe of your choice
  • Temperature gradients and setpoints possible
  • For best product stability results

*protected by RoSS.KSET and designed to your preferred bag

Our customers trust in us:

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  • Graph showing temperature change over time with three different bags.

  • Controlled rate freezer for cryopreservation with RoSS.LN2F

    The left-side graph illustrates the outcomes of a controlled freeze run with RoSS.LN2F conducted at a cooling rate of -1.15°C/min​. Employing a controlled freezing rate leads to markedly enhanced cell viability and recovery compared to uncontrolled freezing. 

    • Cooling/freezing rates in bags are adjustable from 1k/min to 23k/min (chamber temperature)
    • Starting from LPTF (last point to freeze) to -170°C
    • Fast cooling of chamber and subsequent continuous LN2 injection (makes system controllable and economically in operations)
    • Adjustable phase transition time for higher product safety

Controlled rate freezing of cells

Along with autoclaves, centrifuges, laboratory freezers, incubators, refrigerators and ultra-low temperature freezers (ult freezers), cryostorage and (consequently) cryogenic freezers have become indispensable as lab equipment for many researchers, healthcare providers and drug product manufacturers.

Liquid nitrogen freezers like the RoSS.LN2F are used when stem cells, gene therapies, cell culture microplates or several reagents and new products need to be prepared for long-term storage under cryogenic temperatures.

Sample storage of a few vials or pipettes is equally important as storing greater amounts of biologics by the means of a -170°C cryogenic freezer.

Not only for shippers, storage capacity in liquid nitrogen storage is essential, since cost effectiveness and high efficiency are necessary to be achieved. Furthermore, user-friendliness in terms of easy access or intuitive operation via touch screen are also factors to be considered when choosing the right cryogenic freezing device.

Downloads

Preview of datasheet for RoSS.LN2F cryogenic freezer by Single Use Support
Datasheet: RoSS.LN2F
Preview of datasheet for RoSS.LN2F cryogenic freezer by Single Use Support
Datasheet: RoSS.LN2F
 
Preview of Single Use Support's study about cell viability
Study: Increased CHO Cell Viability with Controlled Liquid Nitrogen Freezer
Preview of Single Use Support's study about cell viability
Study: Increased CHO Cell Viability with Controlled Liquid Nitrogen Freezer
 
Preview of Single Use Support's application note about controlled filling and freezing of cells
App Note: "Bestcellers": Controlled Filling & Freezing of Cells
Preview of Single Use Support's application note about controlled filling and freezing of cells
App Note: "Bestcellers": Controlled Filling & Freezing of Cells
 

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More information

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Stressing Cells: The Role of Cryoprotectants in ATMP Cryopreservation

In cell and tissue therapies, living cells are used to treat previously chronic diseases, like cancer forms, arthritis, or certain autoimmune disorders. To be effective, the living therapeutic product requires administration with a sufficient c...

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Study: Optimizing Cell Viability During CHO Cell Freezing

Study: Optimizing Cell Viability During CHO Cell Freezing

Improving Cryogenic Freezing of a Mammalian Cell Line A study was conducted aiming to optimize the cryogenic freezing process of CHO-K1 cell line using an innovative controlled rate liquid nitrogen freezer (RoSS.LN2F). The mammalian cells were fil...

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Controlling cryogenic freezing with liquid nitrogen

Controlling cryogenic freezing with liquid nitrogen

Cryogenic freezing is a necessary process for the preservation of molecules, cells, genetic material, blood, and tissues. Single-use technologies enable protection of such substances from contamination and failure and preserve them for long-term s...

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FAQ about cryogenic freezing

What is a cryogenic freezer?

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A cryogenic freezer is a device that is made to freeze goods like certain biological down to extremely low – cryogenic – temperatures. Often, liquid nitrogen is used to achieve temperatures lower than -150°C and to cryogenically freeze goods.

What is cryopreservation?

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Cryopreservation is a process carried out along the supply chain of certain biologicals in order to maintain their structure and capabilities over time. By the means of cryogenic temperatures, cell metabolism and other processes within a product (like cells, genomic materials, tissue or organs) that would promote its deterioration are slowed/stopped so that it can be stored over time with little to no quality loss.

Cryopreservation requires extremely cold temperatures, lower than -180°C.

How much does a cryogenic freezer cost?

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Although the price range for cryogenic freezers is wide, one can state that they are usually more expensive than regular freezers (that cannot achieve these low temperatures), given the fact that cryopreservation is a highly complex procedure. Therefore, several safety mechanisms need to be in place, as well as measures that must be taken to avoid contamination.

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