Single Use Support's end-to-end process solutions facilitate high density cell banking (HDCB) for seed train intensification. The high degree of automated process steps in cell aliquotation and cryopreservation help maximize cell viability, resulting in increased process efficiency and greater patient safety.
Seed train intensification is a transformative approach to cell culture expansion, allowing to reduce production timelines, costs and risks of contamination. It allows scientists, researchers and manufacturers to rapidly and efficiently develop a number of cell lines for the inoculation of bioreactors used in production. To get the train rolling, multiple optimized process units must be combined to enable high cell density cultivation & efficient high cell density cryopreservation:
Automated aseptic filling platforms, such as RoSS.FILL , enable accurate, fast and safe dispensing into small single-use bags. Introducing 21 CFR Part 11 and GMP-compliance paired with high filling accuracy help advance fluid management processes. IRIS single-use assemblies are customized off-the-shelf fluid transfer tubing sets to provide an aseptically closed system and qualified fluid path.
Before filling cells into single-use bags, it is important to ensure that the cell count is consistent from bag to bag. Homogenizing devices, like RoSS.PADL, ensure cooling of high density cells after DMSO addition and uniform cell count distribution during aliquoting.
Temperature control during freezing and thawing can be achieved through plate-based platforms, e.g. RoSS.pFTU, or controlled-rate blast freezers, such as the cryogenic freezer RoSS.LN2F. Both enable a 21 CFR part 11 compliant automated cold chain process that performs protocol-driven cell freezing at approx. -1°C/min, the optimal cooling rate to maximize cell viability and recovery.
Secondary packaging to protect single-use bags, such as RoSS.KSET, enables a safe cell freezing and storage. With optimal heat transfer, it is an efficient way to standardize scalable cell cryopreservation as a method for banking CHO cells as intermediates.
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High-density cell Banking is a crucial step in the seed train intensification process for biopharmaceutical production. To get the train rolling, multiple optimized process units, that enable high cell densities cultivation & efficient high cell density cryopreservation, need to be combined. Download our free Whitepaper and learn more.
Watch as Brian Moloney and Khalil Essani discuss the process of seed train intensification, the challenges of aliquotating and cryopreserving CHO cells, and how to achieve high cell viability and recovery.
