PLASMID DNA (PDNA)

Illustration of a DNA highlighted mutation in yellow on a blue background

Plasmid DNA (pDNA) Manufacturing

Plasmid DNA (pDNA) is used for a wide range of applications in research, preclinical and clinical studies. Most commonly known in the context of COVID-19 vaccines, pDNA serves as a starting material for the production of mRNA, but also for producing viral vectors, such as AAVs (Adeno-Associated Virus Vectors) and Lentiviral Vectors. 

Using E. coli fermentation, Plasmid DNA is produced in large bioreactors to generate enough biomass to inoculate a larger fermenter, followed by clarification, filtration steps in downstream bioprocessing.

The manufacturing process is typically slow, expensive, has limited capacity, and is vulnerable to batch failure. Flexible and scalable platforms for GMP-compliant plasmid DNA manufacturing improve commercial production.

pDNA Production for Manufacturing mRNA

Plasmid DNA (pDNA) is a critical starting marterial during the mRNA production. Here it is important to consider controlled and reliable processes for aliquotation & filtration,freeze & thaw,ultra cold storage and shipment of your substances between the major production steps of pDNA like Fermentation, Cell Harvest and Clarification and the following mRNA manufacturing processes.

The Chilled Future of RNA Therapeutics Filling & Freezing Applications

The fluid management process for mRNA, pDNA and viral vectors for cryopreservation are often performed manually and in an uncontrolled manner. As a result, they are prone to inefficiencies and increased product loss. Learn how modular and flexible single-use technologies optimize the manufacturing process in the following App Note.

Preview of an app note about filling and freezing of RNA therapeutics

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App Note: The Chilled Future of RNA Therapeutics Filling & Freezing Applications

This application note explores scalable, GMP-compatible solutions for the sterile filling and cryopreservation of mRNA, pDNA, and viral vectors. It presents modular technologies that ensure temperature control, minimize product loss, and support high-accuracy aliquotation - paving the way for efficient commercialization of RNA-based therapeutics.

App Note: The Chilled Future of RNA Therapeutics Filling & Freezing Applications
 

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