Cryogenic Freezer

Cryogenic freezing down to -170°C/-274°F | RoSS.LN2F

RoSS.LN2F is a powerful cryogenic controlled rate freezer for cryogenic freezing down to -170°C/-274°F. An enclosed and innovative platform system for dosed freezing for cell and gene therapies eliminates direct exposure and the need for mechanical compressors. This ensures safe, low maintenance and energy efficient handling.

RoSS.LN2F_Product Photo in Progress-4

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Advantages of the cryogenic freezer

  • Freeze your substance of high value in any single-use bioprocess container*
  • Fully automated & controlled
  • Fully Scalable
  • At highest possible speed & accuracy
  • Any freeze recipe of your choice
  • For best product stability results

*protected by RoSS.KSET and designed to your preferred bag

Controlled cryogenic freezing with RoSS.LN2F

The left-side graph illustrates the outcomes of a controlled freeze run with RoSS.LN2F conducted at a cooling rate of -1.15°C/min​. Employing a controlled freezing rate leads to markedly enhanced cell viability and recovery compared to uncontrolled freezing. 

  • Cooling/freezing rates in bags are adjustable from 0.5K/min to max. 4K/min 
  • Starting from LPTF (last point to freeze) to -170°C/-274°F
  • Cooling/freezing performance based on innovative, controllable LN2 injection system
  • Adjustable phase transition time for higher product safety
  • Innovation is based on the fast cooling of chamber and subsequent continuous LN2 injection (makes system controllable and economically in operations)
RoSS LN2F cryogenic freezer example freeze curve

Increased CHO Cell Viability with Controlled Liquid Nitrogen Freezer

The study was conducted aiming to optimize the cryogenic freezing process of the mammalian CHO-K1 cell line with an innovative controlled rate liquid nitrogen freezer (RoSS.LN2F). It incorporated three viability assays, namely Trypan, LIVE/DEAD and Fluorescence, to comprehensively assess the impact of varying freezing rates on post-thaw cell recovery.

Study Summary_Optimization of the freezing protocol for CHO-K1 cells

Study: Increased CHO Cell Viability with Controlled Liquid Nitrogen Freezer

Filesize: 0.7 MB – Mime-Type: application/pdf

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Rentschler Biopharma SE



Cryogenic storage as an essential component in modern life sciences

Along with autoclaves, centrifuges, laboratory freezers, incubators, refrigerators and ultra-low temperature freezers (ult freezers), cryostorage and (consequently) cryogenic freezers have become indispensable as lab equipment for many researchers, healthcare providers and drug product manufacturers.

Liquid nitrogen freezers like the RoSS.LN2F, made of stainless steel and with a temperature range that goes down to as low as -170°C/-274°F are used when stem cells, gene therapies, cell culture microplates or several reagents and new products need to be prepared for long-term storage under cryogenic temperatures.

Sample storage of a few vials or pipettes is equally important as storing greater amounts of biologics by the means of a -170°C/-274°F cryogenic freezer.

Not only for shippers, storage capacity in liquid nitrogen storage is essential, since cost effectiveness and high efficiency are necessary to be achieved; furthermore, user-friendliness in terms of easy access or intuitive operation via touch screen are also factors to be considered when choosing the right cryogenic freezing device.

Data sheet


Datasheet RoSS.LN2F

Filesize: 1.04 MB – Mime-Type: application/pdf

App Note_Bestcellers-Controlled Filling Freezing of Cells

App Note: "Bestcellers": Controlled Filling & Freezing of Cells

Filesize: 2.39 MB – Mime-Type: application/pdf

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Cell and gene therapy filling machine with RoSS.FILL CGT system

RoSS.FILL CGT | Fill-Filtration | 1mL-1000mL

1mL-1000mL: Fully automated cell and gene filling machine system for aseptic filling of multiple small single use bags. Special designed for use in cell & gene therapies with batch sizes at low volumes. With its high filling accuracy, the platform provides filling from 36 up to 128+ bags with one or several racks.

RoSS.KSET top view

ROSS.KSET | Protecting small single-use bags

RoSS.KSET offers the protection for drug substances with volumes less than 250 mL. The shell of our CGT bag protection system is robust, closed, safe and sterile – most suitable for cell and gene therapies or clinical studies.

For larger volumes, RoSS can protect one or multiple single-use bags in one shell.

Lab freezer pharma with RoSS.pFTU labscale

RoSS.pFTU Lab-Scale

Compatible with single use bags of all sizes and manufacturers. Our lab scale freezer is the perfect solution for studies conducted in labs and, above all, if you want to start a controlled and scalable freezing process.


RoSS.PADL | Homogenizing Solution

RoSS.PADL is a scalable massaging platform for achieving a uniform mixture in
single-use bags. With integrated cooling and heating, it maintains optimal temperatures

FAQ about cryogenic freezing

What is a cryogenic freezer?

A cryogenic freezer is a device that is made to freeze goods like certain biological down to extremely low – cryogenic – temperatures. Often, liquid nitrogen is used to achieve temperatures lower than -150°C and to cryogenically freeze goods.

What is cryopreservation?

Cryopreservation is a process carried out along the supply chain of certain biologicals in order to maintain their structure and capabilities over time. By the means of cryogenic temperatures, cell metabolism and other processes within a product (like cells, genomic materials, tissue or organs) that would promote its deterioration are slowed/stopped so that it can be stored over time with little to no quality loss.

Cryopreservation requires extremely cold temperatures, lower than -180°C.

How much does a cryogenic freezer cost?

Although the price range for cryogenic freezers is wide, one can state that they are usually more expensive than regular freezers (that cannot achieve these low temperatures), given the fact that cryopreservation is a highly complex procedure. Therefore, several safety mechanisms need to be in place, as well as measures that must be taken to avoid contamination.

More information


Whitepaper: How controlled freezing enables scalability

In a comprehensive study the impact of ice front growth speed on scalability of freezing protein solutions has been evaluated.


Cryogenic Freezing: All you need to know

Cryopreservation is an effective procedure for the quality-preserving storage of many biomaterials. Find out what cryopreservation actually is, what has to be considered along the process and where it is applied – all summed up in this article.

RoSS.pFTU Lab Scale - in process (1 von 9)-min

App Note: Cell Monolayer Cryopreservation

Advanced cell-based assay workflow with CryoLogyx Thaw and Test pre-plated cell and controlled, scalable freezing process solution RoSS.pFTU to achieve effective cryopreservation of cells.

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