RoSS.LN2F is a powerful cryogenic controlled rate freezer for cryopreservation down to -170°C. An enclosed and innovative platform system for dosed freezing for cell and gene therapies eliminates direct exposure and the need for mechanical compressors.
Advantages of cryogenic freezer
- Suitable for any single-use bioprocess container*
- Temperature range: from room temperature to -170°C
- Fully automated & controlled
- At highest speed & accuracy
- Any freeze recipe of your choice
- Temperature gradients and setpoints possible
- For best product stability results
*protected by RoSS.KSET and designed to your preferred bag
Controlled rate freezer for cryopreservation with RoSS.LN2F
The left-side graph illustrates the outcomes of a controlled freeze run with RoSS.LN2F conducted at a cooling rate of -1.15°C/min. Employing a controlled freezing rate leads to markedly enhanced cell viability and recovery compared to uncontrolled freezing.
- Cooling/freezing rates in bags are adjustable from 1k/min to 23k/min (chamber temperature)
- Starting from LPTF (last point to freeze) to -170°C
- Cooling/freezing performance based on innovative, controllable LN2 injection system
- Adjustable phase transition time for higher product safety
- Innovation is based on the fast cooling of chamber and subsequent continuous LN2 injection (makes system controllable and economically in operations)
Increased CHO Cell Viability with Controlled Liquid Nitrogen Freezer
The study was conducted aiming to optimize the cryogenic freezing process of the mammalian CHO-K1 cell line with an innovative controlled rate liquid nitrogen freezer (RoSS.LN2F). It incorporated three viability assays, namely Trypan, LIVE/DEAD and Fluorescence, to comprehensively assess the impact of varying freezing rates on post-thaw cell recovery.
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Controlled rate freezing of cells
Along with autoclaves, centrifuges, laboratory freezers, incubators, refrigerators and ultra-low temperature freezers (ult freezers), cryostorage and (consequently) cryogenic freezers have become indispensable as lab equipment for many researchers, healthcare providers and drug product manufacturers.
Liquid nitrogen freezers like the RoSS.LN2F, made of stainless steel and with a temperature range that goes down to as low as -170°C are used when stem cells, gene therapies, cell culture microplates or several reagents and new products need to be prepared for long-term storage under cryogenic temperatures.
Sample storage of a few vials or pipettes is equally important as storing greater amounts of biologics by the means of a -170°C cryogenic freezer.
Not only for shippers, storage capacity in liquid nitrogen storage is essential, since cost effectiveness and high efficiency are necessary to be achieved; furthermore, user-friendliness in terms of easy access or intuitive operation via touch screen are also factors to be considered when choosing the right cryogenic freezing device.
More solutions
FAQ about cryogenic freezing
What is a cryogenic freezer?
A cryogenic freezer is a device that is made to freeze goods like certain biological down to extremely low – cryogenic – temperatures. Often, liquid nitrogen is used to achieve temperatures lower than -150°C and to cryogenically freeze goods.
What is cryopreservation?
Cryopreservation is a process carried out along the supply chain of certain biologicals in order to maintain their structure and capabilities over time. By the means of cryogenic temperatures, cell metabolism and other processes within a product (like cells, genomic materials, tissue or organs) that would promote its deterioration are slowed/stopped so that it can be stored over time with little to no quality loss.
Cryopreservation requires extremely cold temperatures, lower than -180°C.
How much does a cryogenic freezer cost?
Although the price range for cryogenic freezers is wide, one can state that they are usually more expensive than regular freezers (that cannot achieve these low temperatures), given the fact that cryopreservation is a highly complex procedure. Therefore, several safety mechanisms need to be in place, as well as measures that must be taken to avoid contamination.
