Simplifying Cryopreservation of Cell Monolayers

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Brian Moloney

August 9, 2022

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Cell monolayers are an ideal method and widely used in the discovery of new drug compounds and the study of cell biology. Until now, there was no cryopreservation technology available to store cell banks pre-plated commercially. The conventional approach of cell-based assay workflow was time- and resource-consuming. Now, the cryoprotectant technology CryoShieldTM from CryoLogyx together with the controlled and scalable plate freeze-thaw platform RoSS.pFTU from Single Use Support, mark a revolution in commercial cell banking: Cryopreserved Thaw and Use Cell Plates improve the process of preparing cell cultures to carry out cell-based experiments to a large extent. 

Study results confirm a consistent and uniform high ratio of live cell recovery after cryopreservation of cells with accelerated cell-based process workflow. 

Convenient, ready to use from the freezer, cell-based assays could accelerate and simplify the discovery of pharmaceutically active compounds, biocompatibility testing, assay development and discovery of cell signalling and disease pathways.

Brian Moloney

Labs benefit greatly from on demand access to cell-based assays by simplified workflows and highest flexibility. This on demand access to cell-based assays increases significantly the range and amount of experiments they can carry out in the same time.

Read the application note to learn more about the effect of controlled and scalable freezing to achieve advanced process solutions with cryopreserved pre-plated cell monolayers. 

App Note: Cell Monolayer Cryopreservation

‘Thaw-and-Use Assay Cells’ made using CryoShieldTM and Single Use Support‘s technologies are a highly effective solution for a range of cell-based assays, from transfection studies to running toxicity screening experiments for drug candidates. No specialised equipment is required by the end user for the thawing procedure, they simply remove the protective film, add cell media, and incubate overnight. Overall, we have shown that cells can be cryopreserved as ‘assay-ready’ monolayers, in a scalable and versatile format. This will enable the development of new screening technologies and enhance automation as well as reduce the experimental burden on researchers, and ultimately enable the highest quality models to be made available to anyone.
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Brian Moloney

Director Key Account Management

Brian Moloney is Director Key Account Management at Single Use Support. With this role he is responsible for leading and enhancing strategic client relationships and driving customer satisfaction by aligning our products with client needs. Based on his extensive expertise as service, product and project manager at GE Power, Brian is now market expert in pharmaceutical fluid management. He has joined Single Use Support in 2021.

Find articles from Brian Moloney on Medium: https://medium.com/@brian-moloney

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