Viral Vectors

Viral Vector Production

With the discovery of new fields of application for viral vectors in gene therapy comes an increased need for dedicated solutions in manufacturing and supply chain. Single Use Support has a wide product portfolio ready to meet manufacturers’ needs for:

  • Primary packaging (suitable for cryopreservation)
  • Fluid management Solutions
  • Cryogenic Freezers 
  • Ultra-Cold Storage Freezers
  • Shipping Containers

They will help you navigate the challenges and pitfalls to facilitate the medical breakthroughs made possible by viral vectors.

viral vectors gene therapy 3 aav

Most common Viral Vectors

Automated and modular single-use technology solutions bring a variety of advantages viral vector manufacturing processes, such as a high level of safety and cost and resource efficiencies. The most common viral vectors our products are used for are:

  • AVVs – adenoviral vectors, i.e. vectors based on adenoviruses
  • AAVsadeno-associated viral vectors (AAV vectors)
  • LVVs – lentiviral vectors, i.e. vectors based on lentiviruses
  • RVVs – retroviral vectors, i.e. vectors based on retroviruses

Scalable Single-Use Technologies for Viral Vector Processes


Primary Packaging


Feed Source for Aliquotation


Automated Aliquotation

Protection for small single use bags CGT with RoSS.KSET

Robust Protection

Plate freezer Biopharma large scale with RoSS.pFTU

Controlled Freezing


Cold Storage

Shipping drug substances in single-use bag containers safely with RoSS.SHIP


Primary Packaging

IRIS Single-Use Bag (2D or 3D)

Store and ship anything from cell culture samples to large drug substance volumes reliably and on time. 2D or 3D single-use bioprocess containers in any size.

Single-use bioprocess container by Single Use Support

Feed Source for Aliquotation


RoSS.PADL is a platform to standardize cooling of your single-use bag whilst gently massaging it to ensure a homogeneous mixture of your substance.

RoSS.PADL Product

Automated Aliquotation


With RoSS.FILL, bags can be filled at high throughput and unprecedented filling accuracy in a fully automated manner. RoSS.FILL is compatible to all single use bags and bottles and can be scaled up to more than 300L filling volume or scaled out to 100+ smaller bags.

Automated filling and draining system for CGT - RoSS.FILL CGT

RoSS.FILL Lab Scale

Accuracy is key: Fully automated platform system for aseptic filling of up to 12 single-use bags in standard configuration and is extendable to higher bag quantities. Most suitable for filling small volumes in cell & gene therapies or seed train intensification.

RoSS.FILL CGT Lab Scale_product photo (2 von 10)


RoSS.FILL Bag can dispense up to 300L in 1 hour in a fully automated manner. RoSS.FILL is compatible to all single use bags and bottles and can include filtration and PUPSIT prior to aliquotation.

Aseptic bag filling machine RoSS.FILL

Robust Protection


Single use bags with a volume of less than 250ml are covered with a first layer of soft 3D foam which is complemented by an innovative and intuitive locking mechanism. Its robust stainless steel frame protects the single use bag during freezing and shipping.

RoSS.KSET_Unfrozen (1 von 5)

RoSS® Shell

With the RoSS Shell system, the sensitive single use bag is covered in 3D foam, which is held by a highly robust plastic frame. Bottom and top are made of stainless steel – ideal for the freezeing process and absolutely robust.

RoSS shell protecting single use bags open

Controlled Freezing

Controlled Plate-Based Freezer (-80°C)

The freeze & thaw system RoSS.pFTU is a fully automated and scalable plate-based freezing unit. Both economically and in terms of product stability this cGMP compliant platform enables full control over freezing with optional connection to your MES. The plate freezer RoSS.pFTU Mid Scale is adapted to single-use bags in RoSS shells and RoSS.KSET with smaller volumes of 1L and less for a batch sizes of up to 100L.

Plate freezer biopharma with RoSS.pFTU midscale

RoSS.LN2F | Cryogenic Freezer

RoSS.LN2F is a powerful cryogenic controlled rate freezer for temperatures down to -180°C/-292°F. An enclosed and innovative platform system for dosed freezing for cell and gene therapies enables no direct exposure, and no mechanical compressors are needed. This ensures a safe, low-maintenance and energy-saving handling.

RoSS.LN2F_Product Photo in Progress-4

Lab Scale Controlled Plate-Freezer (-80°C)

Controlled freezeing and thawing of small batches - more important than ever for cell & gene therapies and clinical studies! The plate-based freeze & thaw platform for labs RoSS.pFTU Lab Scale is the perfect solution for one or multiple single use bags of up to in total 10L. GMP-compliant computerized system optional.

RoSS.pFTU Lab Scale_Product Photo

Large Scale Controlled Plate-Freezer (-80°C)

The freeze & thaw system RoSS.pFTU is a fully automated and scalable plate-based freezing unit. Both economically and in terms of product stability we have reached the next level. The plate-based platform RoSS.pFTU Large Scale is completely adapted to RoSS shell system for a batch sizes of up to 400L and more.

Plate freezer biopharma large scale freezing with RoSS.pFTU largescale

Cold Storage

RoSS.FRDG | Cold Storage

Ultra-Cold Storage for frozen drug substances in different sizes. RoSS.FRDG keeps the desired set point temperature down to -80°C.

Ultra-cold storage RoSS.FRDG



Storage at -80°C/-150°C & shipment. 100% protection from external mechanical influences and different possible sizes will fulfil your individualized need. Also monitoring temperature and location-tracking are no problems.


Learn more about navigating these 5 overlooked challenges in Viral Vector Manufacturing with regards to fluid management challenges thanks to modular and flexible single-use technologies:

  • Scalability in Cell Line Development 
  • Filling Accuracy in Bag Aliquoting
  • Viscosity in Liquid Transfer
  • Product Viability after Freezing & Thawing and Cryopreservation
  • Bag Breakages in viral vector storage and shipping
Guide_Navigating 5 Overlooked Challenges in Viral Vector Manufacturing_Single Use Support

Navigating 5 Overlooked Challenges in Viral Vector Manufacturing

Filesize: 1.21 MB – Mime-Type: application/pdf

More Information

The fluid management process for mRNA, pDNA and viral vectors for cryopreservation are often performed manually and in an uncontrolled manner. As a result, they are prone to inefficiencies and increased product loss. Learn how modular and flexible single-use technologies optimize the manufacturing process in the following App Note.

Gene therapy manufacturing-5 requirements to scale up

Gene therapy manufacturing - 5 requirements to scale up

Gene therapies have become a highly promising field of groundbreaking next generation treatment, but there are some challenges to face within the manufacturing process. Here are 5 factors that will be crucial for upscaling gene therapy manufacturing with single-use technology.


AAV production & efficiency improvements with single-use technology

With adeno-associated virus being a popular viral vector, along with a growing interest in gene therapies, there is an increased need for cost-effective vector manufacturing technologies and viral vector production platforms for human gene therapy products.

Controlled Plate-Freezer_1

Freezing & thawing viral vectors: Best practices

Proper handling of viral vectors during storage, freezing, and thawing is crucial to maintaining their integrity and efficacy. In this article, we will explore best practices for preserving viral vectors, ensuring their stability, and maximizing their potential for various applications.

RoSS.PADL Homogenizing Solution for Aliquot-to-Aliquot consistency

Aliquotation & homogenization of viral vectors

In this article, we uncover the significance of a professional aliquotation process, the challenges entailed in filling viral vectors like AAVs, and the innovative solutions supporting to master those challenges.

Viral vector simply explained

Viral vector simply explained

Viral vectors have become an indispensable tools in therapeutic and research applications. In this article, we will provide a broad overview of this fascinating topic.

Viral vector manufacturing: Production methods & future advances

Viral vector manufacturing: Production methods & future advances

Viral vector manufacturing involves advanced biotechnological methods, with scalability being one of the major difficulties. In this article, we will discuss both challenges and innovative approaches for viral vector production.

Safe handling AAVs

Safe handling & Storage of Viral Vectors

Viral vectors like Adeno-associated viruses (AAVs) are popular vectors for gene therapy and vaccines. And yet, there is product loss due to leakages of primary packagings or other mishandling of fluids. In order to deliver high-quality and viable products efficiently, safe handling experience a growing interest.