Study: Increased CHO Cell Viability with Controlled Liquid Nitrogen Freezer
Study: Increased CHO Cell Viability with Controlled Liquid Nitrogen Freezer
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Increasing cell viability of a mammalian cell line with controlled rate freezing
The study was conducted aiming to optimize the cryogenic freezing process of the mammalian CHO-K1 cell line with an innovative controlled rate liquid nitrogen freezer (RoSS.LN2F). Cryopreservation of cells is absolutely crucial in all biotechnological processes to maintain critical biological functions of producer strains. In this study three viability assays, namely Trypan Blue, LIVE/DEAD and Fluorescence, were incorporated to comprehensively assess the impact of varying freezing rates on post-thaw cell recovery.