Freeze & Thaw
April 10, 2024
Protein storage: How to increase protein stability?
Prior to be used in diagnostics or therapeutics, proteins have to be safely stored. In this article, we will assess methods to preserve their stability upon storage.
April 9, 2024
Protein stability – all you need to know
Protein stability is crucial to be maintained along the manufacturing process of protein-based products. In this article, we will discover what influences protein stability, along with methods to assess and increase it.
March 21, 2024
Cold Storage Requirements for Active Pharmaceutical Ingredients
Active pharmaceutical ingredients (APIs) are fundamental for drug products to be effective, but require special treatment – e.g. considering humidity, light, or temperature. In this article, we will highlight the different cold storage requirements of APIs, including examples and an overview of different practical approaches.
February 8, 2024
Stressing Cells: The Role of Cryoprotectants in ATMP Cryopreservation
The objective of an optimal cryopreservation strategy is to deactivate (put on hold) degenerative cellular pathways and preserve proliferative potential by reducing the temperature below –130°C. To reduce the effects of ice crystal and osmotic injuries, cryopreservation protocols usually incorporate cryoprotective agents (CPAs).
November 20, 2023
Advancements in Small Volumes Drug Freezing Techniques
Freezing small volumes extends beyond laboratory settings to include commercialized manufacturing processes for small batches in biomanufacturing, like cell banking, gene therapies utilizing viral vectors, lipid nanoparticles, and fill & finish procedures.
September 6, 2023
Sustainable Freezing in Biopharma: An Exploration of Natural Gas Usage
In the biopharmaceutical sector, the utilization of F-Gases is on a decline. However, the need for cryopreservation of drug substances will remain. In order to contribute to a more environmentally friendly freezing of drugs, more sustainable technologies need to be developed and applied. But is sustainable freezing even possible?
August 11, 2023
Cryopreservation techniques & best practice freezing methods
The preservation of cells at cryogenic temperature can be achieved using different techniques. This is far from a simple freezing process; it's a carefully controlled method that prevents the formation of ice crystals, thus maintaining the integrity of the biological material.
August 11, 2023
How to prepare cells for cryopreservation?
This guide provides an overview of the necessary steps and considerations for preparing cells for cryopreservation. Key topics include selecting appropriate cells, cultivating and harvesting them under controlled conditions, utilizing proper cryoprotective agents, and applying specific freezing techniques.
August 9, 2023
Thawing cells – process, difficulties & recommendations
Cell thawing is a critical procedure in the area of life science and medicine. Ensuring a gentle thawing of frozen cells is essential for preserving their viability and functionality. In this article, we will highlight the cell thawing process, discuss common challenges, and provide recommendations for achieving optimal results in cell banking.
July 28, 2023
Cryovessels in Biopharmaceutical Manufacture: When Does the Tank Run Out?
It is many decades since cryovessels emerged as a groundbreaking solution in the biopharmaceutical industry, enabling the safe and efficient storage of bulk drug substances. However, the introduction of plate-based freezing methods has further advanced the technology available for the freezing of biopharmaceuticals, offering improved product quality and consistency.
July 28, 2023
Lyophilization considerations: Comparing freeze-drying to freezing for biopharmaceutical products
Not all biopharmaceuticals are suitable for freeze-drying. Depending on the specific stability requirements the preservation of biopharmaceuticals is either with cryopreservation or lyophilization.
June 23, 2023
Evolving Methods of Bulk Freezing
New technologies, sauch as plate-based freezing and thawing based on single-use systems, improve process efficiency and product quality. Innovative solutions are breaking the ice for the evolution of bulk drug freezing.