Freeze & Thaw

Discover everything you need to know about freeze & thaw processes in our detailed articles. These include the freezing process to sub-zero temperatures, the cold storage and cold shipping of biopharmaceuticals. Learn about different methods, best practices, and advanced cryopreservation techniques to ensure the highest quality and stability of your biopharmaceutical products. Explore how our innovative freezing solutions are applied across various areas, including cell banking, monoclonal antibody preservation, and bioconjugate stability. 

Newsletter subscription

Get the latest delivered directly to your inbox!

Get the latest delivered directly to your inbox! You can unsubscribe at any time, no hard feelings. Privacy policy.

All articles about Freeze & Thaw

Freeze & Thaw

Interview with NIBRT: Innovating Freezing & Thawing in Bioprocessing

The collaboration between Single Use Support and the National Institute for Bioprocessing Research and Training (NIBRT) exemplifies a shared commitment to advancing scientific knowledge and driving innovation in the bioprocessing industry. 

micheal-eder

Michael Eder

August 13, 2024

Freeze & Thaw

Advancements in Small Volumes Drug Freezing Techniques

Freezing small volumes extends beyond laboratory settings to include commercialized manufacturing processes for small batches in biomanufacturing, like cell banking, gene therapies utilizing viral vectors, lipid nanoparticles, and fill & finish procedures.

Alexander Fuchs | Single Use Support

Alexander Fuchs

November 20, 2023

Freeze & Thaw

Sustainable Freezing in Biopharma: An Exploration of Natural Gas Usage

In the biopharmaceutical sector, the utilization of F-Gases is on a decline. However, the need for cryopreservation of drug substances will remain. In order to contribute to a more environmentally friendly freezing of drugs, more sustainable technologies need to be developed and applied. But is sustainable freezing even possible?

Alexander Fuchs | Single Use Support

Alexander Fuchs

September 6, 2023

Freeze & Thaw

Cryovessels in Biopharmaceutical Manufacture: When Does the Tank Run Out?

It is many decades since cryovessels emerged as a groundbreaking solution in the biopharmaceutical industry, enabling the safe and efficient storage of bulk drug substances. However, the introduction of plate-based freezing methods has further advanced the technology available for the freezing of biopharmaceuticals, offering improved product quality and consistency.

daniel-tischler

Daniel Tischler

July 28, 2023

Freeze & Thaw

Lyophilization considerations: Comparing freeze-drying to freezing for biopharmaceutical products

Not all biopharmaceuticals are suitable for freeze-drying. Depending on the specific stability requirements the preservation of biopharmaceuticals is either with cryopreservation or lyophilization.

micheal-eder

Michael Eder

July 28, 2023

Freeze & Thaw

Freezing mRNA: best practice

Considering the manifold applications of mRNA, it is essential to establish optimal freezing processes that maintain viability and effectiveness. In this article, we will compare different freezing methods for mRNA.

Alexander Fuchs | Single Use Support

Alexander Fuchs

July 4, 2023

Freeze & Thaw

Evolving Methods of Bulk Freezing

New technologies, sauch as plate-based freezing and thawing based on single-use systems, improve process efficiency and product quality. Innovative solutions are breaking the ice for the evolution of bulk drug freezing.

micheal-eder

Michael Eder

June 23, 2023

Freeze & Thaw

Dry to Die: How Spray Drying can be Replaced by Freezing in the Production of Bulk Intermediates

Spray drying often leads to product losses of mAbs or vaccines of more than 30%. Plate-based freezing and thawing solutions enable controlled handling of bulk intermediates safely in a vendor-independent, modular and scalable fluid and cold chain management process. 

daniel-tischler

Daniel Tischler

April 12, 2023

Freeze & Thaw

Shipping pharmaceutical liquids and its challenges

The global distribution of pharmaceuticals is complex and demanding, requiring precise handling and storage of sensitive products. With the rise of personalized medicine and the increasing globalization of the industry, the need for safe and efficient shipping and supply chain has never been more important.

Claus Exenberger - Single Use Support

Claus Exenberger

February 28, 2023

Freeze & Thaw

Best choice for cold storage of Biopharmaceuticals - A Comparison

What's the best choice for long-term cold storage of valuable drug substance? Comparing different Biopharma technologies.

Alexander Fuchs | Single Use Support

Alexander Fuchs

January 3, 2023

Freeze & Thaw

How Hybrid Freeze-Thaw Platforms Defy Cryoconcentration

Freezing and Thawing drug substances in both primary packagings, single-use bags and bottles, in one platform is possible with hybrid freezers. Biopharmaceutical manufacturers and CMOs face challenges to prevent the effect of cryoconcentration during freezing, leading to loss of product quality. Tests have shown that hybrid freezer defy cryoconcentration with homogeneous freezing of both single-use bags and bottles.

daniel-tischler

Daniel Tischler

December 7, 2022

Freeze & Thaw

Controlling cryogenic freezing with liquid nitrogen

To control cryogenic freezing is not an easy task, especially when liquid nitrogen is involved. To keep small molecules chemically stable and prevent chemical degradation, the right practices have to be in place. Find out everything about controlled cryogenic freezing with LN2 in this article!

daniel-tischler

Daniel Tischler

November 8, 2022

Discover more topics

Articles by Manufacturing Processes:

Recommended Downloads

E-Book

Whitepaper: Freezing & Storage of Biopharmaceuticals at -80°C

In this publication we show that the initial price can be deceiving and there are significant additional costs during ongoing usage in the long term. Plate freezers ensure a complete, consistent, and fast freezing (and thawing) to ensure the quality of your high-valued drug substance is maintained, and through simple lifecycle analysis they outpace conventional options regarding total costs.

E-Book

Whitepaper: How Controlled & Scalable Freezing Becomes Reality

Scalability around freezing means the ability to guarantee constant freezing kinetics on proteins (biopharmaceutical molecules) in all scales, filling volumes and loading scenarios. This guide covers how to achieve scalable freezing & thawing of biopharmaceuticals by showing What is scalability? Setup for controlled icefront growth speed Scalability results Outcomes

E-Book

Study: Increased CHO Cell Viability with Controlled Liquid Nitrogen Freezer

The study was conducted aiming to optimize the cryogenic freezing process of the mammalian CHO-K1 cell line with an innovative controlled rate liquid nitrogen freezer (RoSS.LN2F). The study incorporated three viability assays to comprehensively assess the impact of varying freezing rates on post-thaw cell recovery | Trypan blue | LIVE/DEAD | Fluorescence.