Freeze & Thaw

February 8, 2024

Stressing Cells: The Role of Cryoprotectants in ATMP Cryopreservation

Khalil Essani - Single Use Support
Khalil Essani

The objective of an optimal cryopreservation strategy is to deactivate (put on hold) degenerative cellular pathways and preserve proliferative potential by reducing the temperature below –130°C. To reduce the effects of ice crystal and osmotic injuries, cryopreservation protocols usually incorporate cryoprotective agents (CPAs).

November 20, 2023

Advancements in Small Volumes Drug Freezing Techniques

Alexander Fuchs | Single Use Support
Alexander Fuchs

Freezing small volumes extends beyond laboratory settings to include commercialized manufacturing processes for small batches in biomanufacturing, like cell banking, gene therapies utilizing viral vectors, lipid nanoparticles, and fill & finish procedures.

September 6, 2023

Sustainable Freezing in Biopharma: An Exploration of Natural Gas Usage

Alexander Fuchs | Single Use Support
Alexander Fuchs

In the biopharmaceutical sector, the utilization of F-Gases is on a decline. However, the need for cryopreservation of drug substances will remain. In order to contribute to a more environmentally friendly freezing of drugs, more sustainable technologies need to be developed and applied. But is sustainable freezing even possible?

August 11, 2023

Cryopreservation techniques & best practice freezing methods

BRMO
Brian Moloney

The preservation of cells at cryogenic temperature can be achieved using different techniques. This is far from a simple freezing process; it's a carefully controlled method that prevents the formation of ice crystals, thus maintaining the integrity of the biological material.

August 11, 2023

How to prepare cells for cryopreservation?

BRMO
Brian Moloney

This guide provides an overview of the necessary steps and considerations for preparing cells for cryopreservation. Key topics include selecting appropriate cells, cultivating and harvesting them under controlled conditions, utilizing proper cryoprotective agents, and applying specific freezing techniques.

August 9, 2023

Thawing cells – process, difficulties & recommendations

BRMO
Brian Moloney

Cell thawing is a critical procedure in the area of life science and medicine. Ensuring a gentle thawing of frozen cells is essential for preserving their viability and functionality. In this article, we will highlight the cell thawing process, discuss common challenges, and provide recommendations for achieving optimal results in cell banking. 

July 28, 2023

Cryovessels in Biopharmaceutical Manufacture: When Does the Tank Run Out?

daniel-tischler
Daniel Tischler

It is many decades since cryovessels emerged as a groundbreaking solution in the biopharmaceutical industry, enabling the safe and efficient storage of bulk drug substances. However, the introduction of plate-based freezing methods has further advanced the technology available for the freezing of biopharmaceuticals, offering improved product quality and consistency.

July 28, 2023

Lyophilization considerations: Comparing freeze-drying to freezing for biopharmaceutical products

micheal-eder
Michael Eder

Not all biopharmaceuticals are suitable for freeze-drying. Depending on the specific stability requirements the preservation of biopharmaceuticals is either with cryopreservation or lyophilization.

June 23, 2023

Evolving Methods of Bulk Freezing

micheal-eder
Michael Eder

New technologies, sauch as plate-based freezing and thawing based on single-use systems, improve process efficiency and product quality. Innovative solutions are breaking the ice for the evolution of bulk drug freezing.

April 12, 2023

Dry to Die: How Spray Drying can be Replaced by Freezing in the Production of Bulk Intermediates

daniel-tischler
Daniel Tischler

Spray drying often leads to product losses of mAbs or vaccines of more than 30%. Plate-based freezing and thawing solutions enable controlled handling of bulk intermediates safely in a vendor-independent, modular and scalable fluid and cold chain management process. 

March 16, 2023

Filling & Freezing large volumes for fermentation in less than 8 hours

daniel-tischler
Daniel Tischler

The filling and freezing of large volumes for fermentation is an essential part during different production steps in upstream and downstream processing.  In the following, we will explain why it is important to act fast and how an optimization of the process can be achieved. 

March 14, 2023

Cold chain handling for fermentation: Single-use solutions to lower costs

Alexander Fuchs | Single Use Support
Alexander Fuchs

As expert for cold chain handling in bioprocessing, Single Use Support is providing solutions to meet challenges for safe storing and shipping associated with right freezing and thawing methods and temperature control for long-term storage. Their development approach leads to innovative systems that enable manufacturers to replace manual processes with fully automated end-to-end processes that not only push efficiency but also increase output.