All articles

Cell & Gene Therapy

Plasmid DNA manufacturing: The process simply explained

The pDNA manufacturing process is multifaceted yet essential in the biopharmaceutical industry. Here, we will describe the process step by step.

michael-muehlegger

Michael Mühlegger

September 12, 2023

Cell & Gene Therapy

What is a plasmid?

Plasmids are part of the genetic material in bacteria, complementing chromosomal DNA with additional functions. But there is much more to plasmids, as you can read in this article.

michael-muehlegger

Michael Mühlegger

September 7, 2023

Cell & Gene Therapy

Plasmid DNA – 7 facts about pDNA

Plasmid DNA is not only found in bacteria, but also ubiquitous in biotechnology. In this article, we have collected 7 interesting facts on pDNA.

michael-muehlegger

Michael Mühlegger

September 7, 2023

Freeze & Thaw

Sustainable Freezing in Biopharma: An Exploration of Natural Gas Usage

In the biopharmaceutical sector, the utilization of F-Gases is on a decline. However, the need for cryopreservation of drug substances will remain. In order to contribute to a more environmentally friendly freezing of drugs, more sustainable technologies need to be developed and applied. But is sustainable freezing even possible?

Alexander Fuchs | Single Use Support

Alexander Fuchs

September 6, 2023

Cell & Gene Therapy

Revolutionizing Commercial Aliquotation in Advanced Therapies

Single Use Support unveils its latest customized innovation within the product range of automated of biopharmaceutical manufacturing, RoSS.FILL. With its unmatched precision and cutting-edge technology, RoSS.FILL CGT is a game-changer for the cell and gene therapy industry.

BRMO

Brian Moloney

August 22, 2023

Cell Banking

Cryopreservation techniques & best practice freezing methods

The preservation of cells at cryogenic temperature can be achieved using different techniques. This is far from a simple freezing process; it's a carefully controlled method that prevents the formation of ice crystals, thus maintaining the integrity of the biological material.

BRMO

Brian Moloney

August 11, 2023

Cell Banking

How to prepare cells for cryopreservation?

This guide provides an overview of the necessary steps and considerations for preparing cells for cryopreservation. Key topics include selecting appropriate cells, cultivating and harvesting them under controlled conditions, utilizing proper cryoprotective agents, and applying specific freezing techniques.

BRMO

Brian Moloney

August 11, 2023

Cell Banking

Cell viability & viability assays: 7 facts to be aware of

In research, medicine and biotechnology, cell viability is an elementary parameter and Critical Quality Attribute (CQA). In this article, we will present you 7 facts regarding the term, related concepts, improvement possibilities and more.

BRMO

Brian Moloney

August 10, 2023

Cell Banking

Cryopreservation: Purpose, process & applications

Cryopreservation is essential to maintain ex vivo viability of organic materials – indispensable, for instance, in fields like cell banking. In this article, we will explore central aspects of this technique.

BRMO

Brian Moloney

August 10, 2023

Cell Banking

cGMP cell banking: Aliquoting & controlled freezing of cells in less than 1 hour

As the market is constantly evolving, new, fully automated solutions are offered to make GMP cell banking and cGMP cell banking easier to achieve. Single Use Support’s solutions do not only reach out to help make the handling of cell banks safer and more efficient, but also help to support smaller-scale projects through scalable solutions. 

BRMO

Brian Moloney

August 9, 2023

Cell Banking

Thawing cells – process, difficulties & recommendations

Cell thawing is a critical procedure in the area of life science and medicine. Ensuring a gentle thawing of frozen cells is essential for preserving their viability and functionality. In this article, we will highlight the cell thawing process, discuss common challenges, and provide recommendations for achieving optimal results in cell banking. 

BRMO

Brian Moloney

August 9, 2023

Cell Banking

Homogenization of cells & aliquotation in single-use bags

Who can relate to the situation when every cell aliquot is different? That's why cell homogenization and aliquotation are important requirements in modern cell biology research, relevant for scientists and laboratory staff. Scientists and process engineers benefit from uniform cell distribution throughout multiple single-use bioprocess containers for cell banking or other bioprocessing steps.

BRMO

Brian Moloney

August 9, 2023

Recommended Downloads

E-Book

eBook: Filling Gaps in Managing Large Volumes of Biologics

Aseptic aliquotation and cryopreservation of drug substances are crucial process steps in bioprocessing. Even though they’re not in the spotlight of biomanufacturing. In drug development and drug delivery, biologics must be transferred at numerous occasions. Along the journey of monoclonal antibodies, vaccines and more, the drug substance passes lots of process steps where liquid transfer is required. This is relevant for the transitions between upstream processing, downstream processing and fill & finish, but also within these manufacturing milestones. The market of fluid & cold chain management has great growth potential and is on its way to professionalization and industrialization. To meet the challenges of manufacturing drug substances, Single Use Support helps manufacturers and CDMOs to advance their fluid & cold chain management process to achieve a secure and efficient process for liquids on the basis of single-use bags.

E-Book

Whitepaper: How Controlled & Scalable Freezing Becomes Reality

Scalability around freezing means the ability to guarantee constant freezing kinetics on proteins (biopharmaceutical molecules) in all scales, filling volumes and loading scenarios. This guide covers how to achieve scalable freezing & thawing of biopharmaceuticals by showing What is scalability? Setup for controlled icefront growth speed Scalability results Outcomes

E-Book

Whitepaper: Automated Aseptic Filling

Automated Aseptic Filling Unprecedented flexibility to filling well